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Analysis of institutional authors

Vergara-Alert, JAuthorLopez-Serrano, SAuthorPina-Pedrero, SAuthorPerez-Maillo, MAuthorVidal, EAuthorSegales, JAuthorDarji, ACorresponding Author

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April 14, 2019
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Article

Conserved HA-peptide NG34 formulated in pCMV-CTLA4-Ig reduces viral shedding in pigs after a heterosubtypic influenza virus SwH3N2 challenge

Publicated to: PLoS One. 14 (3): e0212431- - 2019-03-01 14(3), DOI: 10.1371/journal.pone.0212431

Authors:

Sisteré-Oró, M; Vergara-Alert, J; Stratmann, T; López-Serrano, S; Pina-Pedrero, S; Córdoba, L; Pérez-Maillo, M; Pleguezuelos, P; Vidal, E; Veljkovic, V; Segalés, J; Nielsen, J; Fomsgaard, A; Darji, A
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Affiliations

2017 SGR 1490 EXOTIQUES. IRTA Investigación y Tecnología Agroalimentarias - Author
2017 SGR 886 ENDEMIQUES. IRTA Investigación y Tecnología Agroalimentarias - Author
2017 SGR 982 ESAD. IRTA Investigación y Tecnología Agroalimentarias - Author
Campus Univ Autonoma Barcelona, IRTA, Ctr Recerca Sanitat Anim CReSA, IRTA UAB, Barcelona, Spain - Author
Campus Univ Autonoma Barcelona, UAB, Ctr Recerca Sanitat Anim CReSA, IRTA UAB, Barcelona, Spain - Author
CReSA M Maeztu 2021 DES. IRTA Investigación y Tecnología Agroalimentarias - Author
Inst Nucl Sci VINCA, Ctr Multidisciplinary Res, Belgrade, Serbia - Author
Producció Animal. IRTA Investigación y Tecnología Agroalimentarias - Author
Sanitat Animal. IRTA Investigación y Tecnología Agroalimentarias - Author
Sanitat Animal. Producció Animal - Author
Statens Serum Inst, Dept Virus & Microbiol Special Diagnost, Virus Res & Dev Lab, Copenhagen S, Denmark - Author
UAB, Fac Vet, Dept Sanitat & Anat Anim, Barcelona, Spain - Author
Univ Barcelona, Fac Biol, Dept Cell Biol Physiol & Immunol, Barcelona, Spain - Author
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Abstract

Swine influenza viruses (SIVs), the causal agents of swine influenza, are not only important to control due to the economic losses in the swine industry, but also can be pandemic pathogens. Vaccination is one of the most relevant strategies to control and prevent influenza infection. Current human vaccines against influenza induce strain-specific immunity and annual update is required due to the virus antigenic shift phenomena. Previously, our group has reported the use of conserved hemagglutinin peptides (HA-peptides) derived from H1-influenza virus as a potential multivalent vaccine candidate. Immunization of swine with these HA-peptides elicited antibodies that recognized and neutralized heterologous influenza viruses in vitro and demonstrated strong hemagglutination-inhibiting activity. In the present work, we cloned one HA-peptide (named NG34) into a plasmid fused with cytotoxic T lymphocyte-associated antigen (CTLA4) which is a molecule that modifies T cell activation and with an adjuvant activity interfering with the adaptive immune response. The resulting plasmid, named pCMV-CTLA4-Ig-NG34, was administered twice to animals employing a needle-free delivery approach. Two studies were carried out to test the efficacy of pCMV-CTLA4-Ig-NG34 as a potential swine influenza vaccine, one in seronegative and another in seropositive pigs against SIV. The second one was aimed to evaluate whether pCMV-CTLA4-Ig-NG34 vaccination would overcome maternally derived antibodies (MDA). After immunization, all animals were intranasally challenged with an H3N2 influenza strain. A complete elimination or significant reduction in the viral shedding was observed within the first week after the challenge in the vaccinated animals from both studies. In addition, no challenged heterologous virus load was detected in the airways of vaccinated pigs. Overall, it is suggested that the pCMV-CTLA4-Ig-NG34 vaccine formulation could potentially be used as a multivalent vaccine against influenza viruses.
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Keywords

a virusantigendna vaccineimmune-responsesimmunizationinfectionprotectionrespiratory-diseaseswine influenzaAbataceptAnimalsDogsHemagglutinin glycoproteins, influenza virusInfluenza a virus, h3n2 subtypeInfluenza vaccinesMadin darby canine kidney cellsMaternally-derived antibodiesOrthomyxoviridae infectionsPeptidesPlasmidsSwineSwine diseasesVaccinationVaccines, dnaVirus shedding

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal PLoS One due to its progression and the good impact it has achieved in recent years, according to the agency Scopus (SJR), it has become a reference in its field. In the year of publication of the work, 2019, it was in position , thus managing to position itself as a Q1 (Primer Cuartil), in the category Multidisciplinary. Notably, the journal is positioned above the 90th percentile.

Independientemente del impacto esperado determinado por el canal de difusión, es importante destacar el impacto real observado de la propia aportación.

Según las diferentes agencias de indexación, el número de citas acumuladas por esta publicación hasta la fecha 2026-04-05:

  • Google Scholar: 9
  • WoS: 8
  • Scopus: 3
  • Europe PMC: 7
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Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2026-04-05:

  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 36 (PlumX).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
  • Assignment of a Handle/URN as an identifier within the deposit in the Institutional Repository: http://hdl.handle.net/20.500.12327/470
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Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: Denmark; Serbia.

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (Sistere-Oro, M) and Last Author (DARJI, AYUB).

the author responsible for correspondence tasks has been DARJI, AYUB.

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Awards linked to the item

This work was funded in part by the Spanish Government, Ministerio de Economia y Competitividad de Espana (MINECO), project: AGL2013-48923-C2-2-R, and by the collaborative infrastructure project funded by the European Comission (EC) under Horizon 2020, project Transvac2-730964-INFRAIA-2016-1. IRTA is supported by CERCA Programme/Generalitat de Catalunya. M.S.-O. is supported by MINECO (scholarship no BES-2014-068506).
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