{rfName}
An

Indexed in

License and use

Icono OpenAccess

Citations

18

Altmetrics

Grant support

This work was supported by the Spanish Ministry of Economy and Competitiveness (SAF2012-38368). Noelia Carmona-Vicente was recipient of a V Segles fellowship from the University of Valencia and Jesus Rodriguez-Diaz was recipient of a Ramon y Cajal contract from the Spanish Ministry of Economy and Competitiveness (RYC-2013-12442). Miren Iturriza-Gomara receives support form the Wellcome Trust ISSF awarded to the University of Liverpool, by the National Institute for Health Research (Grant number NIHR HPRU 2012-10038).

Analysis of institutional authors

Carmona-Vicente, NAuthor

Share

Publications
>
Article

Antibodies against Lewis antigens inhibit the binding of human norovirus GII.4 virus-like particles to saliva but not to intestinal Caco-2 cells

Publicated to:Virology Journal. 13 82- - 2016-05-21 13(), DOI: 10.1186/s12985-016-0538-y

Authors: Carmona-Vicente, N; Allen, DJ; Rodriguez-Diaz, J; Iturriza-Gomara, M; Buesa, J

Affiliations

Abstract

Background: Human noroviruses (NoVs) are the main cause of gastroenteritis worldwide. The most commonly detected NoV strains belong to the genetically diverse GII.4 genotype, with new pandemic variants emerging periodically. Despite extensive efforts, NoV investigation has been hampered by the lack of an effective in vitro cell culture system. However, NoV-derived recombinant virus-like particles (VLPs) resembling empty capsids are good surrogates for analysing NoV antigenicity and virus-ligand interactions. NoV VLPs have been reported to bind to histo-blood group antigens (HBGAs). We have analysed the ability of NoV VLPs derived from GI. 1 genotype and from three GII.4 genotype variants, GII.4-1999, GII.4-2004 and GII.4-2006b, to bind to porcine gastric mucin (PGM), human saliva and differentiated human intestinal Caco-2 cells (D-Caco-2 cells). Results: Distinct patterns of saliva binding with the NoV GII.4 variant VLPs were observed, although they bound to D-Caco-2 cells independently of the expression of HBGAs. Monoclonal antibodies against Lewis antigens were able to block the binding of NoV VLPs to saliva, but not to D-Caco-2 cells. Blocking HBGAs on the surface of D-Caco-2 cells with specific monoclonal antibodies did not affect NoV VLP binding to cellular membranes. Co-localisation of Lewis y (Le(y)) and H-type 2 antigens with NoV VLPs was not observed by immunofluorescence assays. Conclusion: Although the binding of NoV VLPs of GII.4 genotype variants to human saliva samples occur with distinct HBGA binding patterns and can be blocked by antibodies against Lewis antigens, their attachment to D-Caco-2 cells can be mediated by other receptors, which still need further investigation.

Keywords

caco-2 cellsgii.4 genotypehisto-blood group antigens (hbgas)human norovirus (nov)receptor bindingAttachmentBlood group antigensCaco-2 cellsDifferentiationEpithelial-cellsEpochal evolutionExpressionFeline-calicivirusGastroenteritisGii.4 genotypeHisto-blood group antigens (hbgas)Human norovirus (nov)P2 domainReceptor bindingSusceptibilityVirus-like particles (vlps)

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Virology Journal due to its progression and the good impact it has achieved in recent years, according to the agency Scopus (SJR), it has become a reference in its field. In the year of publication of the work, 2016, it was in position , thus managing to position itself as a Q2 (Segundo Cuartil), in the category Infectious Diseases. Notably, the journal is positioned en el Cuartil Q3 for the agency WoS (JCR) in the category Virology.

Independientemente del impacto esperado determinado por el canal de difusión, es importante destacar el impacto real observado de la propia aportación.

Según las diferentes agencias de indexación, el número de citas acumuladas por esta publicación hasta la fecha 2025-06-07:

  • WoS: 8
  • Scopus: 10
  • OpenCitations: 8

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-06-07:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 41.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 41 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 1.5.
  • The number of mentions on the social network X (formerly Twitter): 3 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.

Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: United Kingdom.

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (CARMONA VICENTE, NOELIA) .