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Grant support

This research was funded by the Research Council FORMAS, Stockholm (Project 2017-00946 and Project 2019-00288) and by the Grant RyC2020-028715-I, PID2019-108320RJ-I00, IJCI-2015-24380 and PID2022-136561OB-I00, funded by MCIN/AEI/10.13039/501100011033 (Spain) and FEDER funds (EU).

Analysis of institutional authors

Gardela, JAuthor
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MicroRNA expression in specific segments of the pig periovulatory internal genital tract is differentially regulated by semen or by seminal plasma

Publicated to:Research In Veterinary Science. 168 105134- - 2024-03-01 168(), DOI: 10.1016/j.rvsc.2023.105134

Authors: Alvarez-Rodriguez, Manuel; Martinez-Serrano, Cristina A; Gardela, Jaume; Nieto, Helena; de Mercado, Eduardo; Rodriguez-Martinez, Heriberto

Affiliations

Ctr Recerca Sanitat Anim CReSA, IRTA, Programa Sanitat Anim, Campus Univ Autonoma Barcelona UAB, Barcelona 08193, Spain - Author
Ctr Recerca Sanitat Anim CReSA, Unitat Mixta Invest IRTA UAB Sanitat Anim, Campus Univ Autonoma Barcelona UAB, Barcelona 08193, Spain - Author
Linkoping Univ, Fac Med & Hlth Sci, Dept Biomed & Clin Sci BKV, BKH Obstet & Gynecol, S-58185 Linkoping, Sweden - Author
Sanitat Animal. Producció Animal - Author
Spanish Natl Inst Agr & Food Res & Technol INIA CS, Dept Anim Reprod, Madrid 28040, Spain - Author
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Abstract

microRNAs play pivotal roles during mammalian reproduction, including the cross-talk between gametes, embryos and the maternal genital tract. Mating induces changes in the expression of mRNA transcripts in the female, but whether miRNAs are involved remains to be elucidated. In the current study, we mapped 181 miRNAs in the porcine peri-ovulatory female reproductive tract: Cervix (Cvx), distal and proximal uterus (Dist-Ut, ProxUt), Utero-tubal-junction (UTJ), isthmus (Isth), ampulla (Amp), and infundibulum (Inf) when exposed to semen (natural mating (NM) or artificial insemination (AI-P1)) or to infusions of sperm-free seminal plasma (SP): the first 10 mL of the sperm rich fraction (SP-P1) or the entire ejaculate (SP-E). Among the most interesting findings, NM decreased mir-671, implicated in uterine development and pregnancy loss prior to embryo implantation, in Cvx, Dist-UT, Prox-UT, Isth, and Inf, while it increased in Amp. NM and SP-E induced the downregulation of miRlet7A-1 (Dist-UT, Prox-UT), a regulator of immunity during pregnancy. miR-34C-1, a regulator of endometrial receptivity gene expression, was increased in Dist-UT, UTJ and Amp (NM), in Prox-UT (AI-P1), and in Amp (SPP1). miR-296, a modulator of the inflammatory response and apoptosis, was upregulated in the UTJ (all treatments). NM elicited the highest miRNA activity in the sperm reservoir (UTJ), suggesting that key-regulators such as miR-34c or miR-296 may modulate the metabolic processes linked to the adequate preparation for gamete encounter in the oviduct. Our results suggest that SP should be maintained in AI to warrant miRNA regulation within the female genital tract for reproductive success.

Keywords
ActivationCellsEmbryoEndometriumEpigeneticsFluidGenitalia pigImplantationNcrnaRnasSemen female

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Research In Veterinary Science due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2024 there are still no calculated indicators, but in 2023, it was in position 32/167, thus managing to position itself as a Q1 (Primer Cuartil), in the category Veterinary Sciences.

Independientemente del impacto esperado determinado por el canal de difusión, es importante destacar el impacto real observado de la propia aportación.

Según las diferentes agencias de indexación, el número de citas acumuladas por esta publicación hasta la fecha 2025-05-18:

  • Google Scholar: 2
  • WoS: 2
  • Scopus: 2
  • OpenCitations: 1
Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-05-18:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 9.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 8 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 1.
  • The number of mentions on the social network X (formerly Twitter): 1 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
  • Assignment of a Handle/URN as an identifier within the deposit in the Institutional Repository: http://hdl.handle.net/20.500.12327/2839
Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: Sweden.