{rfName}
Re

Indexed in

License and use

Licencia
Icono OpenAccess

Citations

1

Altmetrics

Grant support

Financial support was provided by the E-RTA2017-00007-00-00 INIA Project (Spain). IRTA-CReSA funds are partially supported by the CERCA programme from the Generalitat de Catalunya.

Analysis of institutional authors

Cobos, AlexAuthorDomingo, MarianoAuthorPerez, MonicaAuthorHuerta, EvaAuthorLlorens, AnnaAuthorSegales, JoaquimCorresponding AuthorSibila, MarinaCorresponding Author

Share

November 25, 2024
Publications
>
Article
Sí

Retrospective investigation of porcine circoviruses in cases of porcine dermatitis and nephropathy syndrome

Publicated to:Veterinary Research. 55 (1): 146- - 2024-11-09 55(1), DOI: 10.1186/s13567-024-01405-8

Authors: Cobos, Alex; Domingo, Mariano; Perez, Monica; Huerta, Eva; Llorens, Anna; Segales, Joaquim; Sibila, Marina

Affiliations

Campus Univ Autonoma Barcelona UAB, Ctr Recerca Sanitat Anim CReSA, Programa Sanitat Anim, Bellaterra 08193, Barcelona, Spain - Author
Campus Univ Autonoma Barcelona UAB, Ctr Recerca Sanitat Anim CReSA, Unitat Mixta Invest IRTA UAB Sanitat Anim, Bellaterra 08193, Barcelona, Spain - Author
Campus Univ Autonoma Barcelona UAB, Dept Sanitat & Anat Anim, Fac Vet, Bellaterra 08193, Barcelona, Spain - Author
WOAH Collaborating Ctr Res & Control Emerging & Re, Bellaterra 08193, Barcelona, Spain - Author

Abstract

Porcine dermatitis and nephropathy syndrome (PDNS) is a severe condition that affects mainly growing pigs and is considered to be caused by a type III hypersensitivity reaction. Although porcine circovirus 2 (PCV-2) is the antigen linked to this condition, porcine circovirus 3 (PCV-3) has also been proposed to be causally associated with PDNS. Moreover, the initial description of porcine circovirus 4 (PCV-4) also related this novel agent to this clinicopathological entity. Therefore, this retrospective study included a large number of PDNS cases (n = 102) fulfilling specific histologic criteria in search of known porcine circoviruses (PCV-1 to PCV-4) by conventional and/or quantitative PCR (qPCR). All the samples were subjected to PCV-2 immunohistochemistry (IHC) or conventional in situ hybridization (C-ISH), and RNAscope (R) (R-ISH) was used to study PCV-2 and PCV-3 localization in a subset of the samples. All PDNS cases were PCV-2 positive by qPCR, while 30 of them (29.4%) yielded PCV-3 qPCR positivity; PCV-2 viral loads were significantly greater than PCV-3 viral loads. All animals were negative for PCV-1 and PCV-4. By C-ISH/IHC, 63 cases (61.8%) were positive for PCV-2, with low to moderate amounts of antigen. R-ISH demonstrated higher sensitivity, as all studied cases were positive; however, neither PCV-2 nor PCV-3 were consistently found within characteristic PDNS lesions. These results indicate that all PDNS-affected pigs were infected with PCV-2, emphasizing the likelihood that this viral antigen is causally linked to this condition. In contrast, no evidence of the association of PCV-1, PCV-3 or PCV-4 with PDNS was found.

Keywords

In situ hybridizationIn-situ hybridizationMultisystemic wasting syndromePigsPorcine circovirus 1 (pcv-1)Porcine circovirus 2 (pcv-2)Porcine circovirus 3 (pcv-3)Porcine circovirus 4 (pcv-4)Porcine dermatitis and nephropathy syndrome (pdns)Real-time quantitative polymerase chain reaction (qpcrSyndrome pdnsSyndrome pmwsSystemic necrotizing vasculitisTissuesViru

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Veterinary Research due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2024 there are still no calculated indicators, but in 2023, it was in position 10/170, thus managing to position itself as a Q1 (Primer Cuartil), in the category Veterinary Sciences. Notably, the journal is positioned above the 90th percentile.

Independientemente del impacto esperado determinado por el canal de difusión, es importante destacar el impacto real observado de la propia aportación.

Según las diferentes agencias de indexación, el número de citas acumuladas por esta publicación hasta la fecha 2025-08-02:

  • WoS: 1

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-08-02:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 1.

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 11.
  • The number of mentions on the social network X (formerly Twitter): 4 (Altmetric).
  • The number of mentions in news outlets: 1 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
  • Assignment of a Handle/URN as an identifier within the deposit in the Institutional Repository: http://hdl.handle.net/20.500.12327/3395

Leadership analysis of institutional authors

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (COBOS ARNALOT, ALEX) and Last Author (SIBILA VIDAL, MARINA).

the authors responsible for correspondence tasks have been SEGALES COMA, JOAQUIM and SIBILA VIDAL, MARINA.