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This work received funding from FEADER and Departament d'Accio Climatica, Alimentacio i Agenda Rural (DACC) of the Generalitat de Catalunya with grant 56 30123 2021 2A from the budget AG06D/482000100/5741/0000. This work also received funding from the European Union's Horizon 2020 research and innovation program under grant agreement No 737089 and grants PID2019-105714RB-I00 and PID2022-136833OB-C21 funded by MICIU/AEI/10.13039/501100011033 and the European Regional Development Fund.

Analysis of institutional authors

Puig-Pujol, AnnaAuthor

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Article

MicroVi: A Cost-Effective Microscopy Solution for Yeast Cell Detection and Count in Wine Value Chain

Publicated to:Biosensors. 15 (1): 40- - 2025-01-12 15(1), DOI: 10.3390/bios15010040

Authors: Benito-Altamirano, Ismael; Moreno, Sergio; Vaz-Romero, David M; Puig-Pujol, Anna; Roca-Domenech, Gemma; Canals, Joan; Vila, Anna; Prades, Joan Daniel; Dieguez, Angel

Affiliations

Altres Activitats. Altres - Author
Catalan Inst Vine & Wine, Inst Agrifood Res & Technol, INCAVI IRTA, Placa Agora 2, Barcelona 08720, Spain - Author
Tech Univ Carolo Wilhelmina Braunschweig, Inst Semicond Technol IHT, Hans Sommer Str 66, D-38106 Braunschweig, Germany - Author
Tech Univ Carolo Wilhelmina Braunschweig, Lab Emerging Nanometrol LENA, Hans Sommer Str 66, D-38106 Braunschweig, Germany - Author
Univ Barcelona, Dept Elect & Biomed Engn, Marti I Franques 1, Barcelona 08028, Spain - Author
Univ Oberta Catalunya, Fac Comp Sci Multimedia & Telecommun, EHlth Ctr, Rambla del Poblenou 156, Barcelona 08018, Spain - Author
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Abstract

In recent years, the wine industry has been researching how to improve wine quality along the production value chain. In this scenario, we present here a new tool, MicroVi, a cost-effective chip-sized microscopy solution to detect and count yeast cells in wine samples. We demonstrate that this novel microscopy setup is able to measure the same type of samples as an optical microscopy system, but with smaller size equipment and with automated cell count configuration. The technology relies on the top of state-of-the-art computer vision pipelines to post-process the images and count the cells. A typical pipeline consists of normalization, feature extraction (i.e., SIFT), image composition (to increase both resolution and scanning area), holographic reconstruction and particle count (i.e., Hough transform). MicroVi achieved a 2.19 mu m resolution by properly resolving the G7.6 features from the USAF Resolving Power Test Target 1951. Additionally, we aimed for a successful calibration of cell counts for Saccharomyces cerevisiae. We compared our direct results with our current optical setup, achieving a linear calibration for measurements ranging from 0.5 to 50 million cells per milliliter. Furthermore, other yeast cells were qualitatively resolved with our MicroVi microscope, such as, Brettanomyces bruxellensis, or bacteria, like, Lactobacillus plantarum, thus confirming the system's reliability for consistent microbial assessment.

Keywords

Ab-on-a-chipChip-sized microscopHolographyWine qualityYeast cell count

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Biosensors due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2025, it was in position 50/141, thus managing to position itself as a Q1 (Primer Cuartil), in the category Nanoscience & Nanotechnology.

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-06-29:

  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 2 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

    It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

    • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
    • Assignment of a Handle/URN as an identifier within the deposit in the Institutional Repository: http://hdl.handle.net/20.500.12327/3592

    Leadership analysis of institutional authors

    This work has been carried out with international collaboration, specifically with researchers from: Germany.