{rfName}
Co

Indexed in

License and Use

Licencia Icono OpenAccess

Citations

1

Altmetrics

Analysis of institutional authors

Martin-Hernandez, AmCorresponding Author

Share

April 25, 2025
Publications
>
Article
Sí

Comprehensive proteomic profiling of Cucumber mosaic virus infection: identifying key proteins and pathways involved in resistance and susceptibility in melon

Publicated to: Bmc Plant Biology. 25 (1): 434- - 2025-04-05 25(1), DOI: 10.1186/s12870-025-06464-3

Authors: Real, N; Garcia-Molina, A; Stolze, SC; Harzen, A; Nakagami, H; Martín-Hernández, AM

Affiliations

Genòmica i Biotecnologia . Producció Vegetal - Author
Inst Recerca & Tecnol Agroalimentaries IRTA, Campus UAB - Author
Max Planck Inst Plant Breeding Res, Carl Von Linne Weg 10 - Author
UB, UAB, CSIC, Ctr Res Agr Genom CRAG,IRTA, C Vall Moronta,Edifici CRAG - Author
See more

Abstract

BackgroundMelon (Cucumis melo L.) is the model species of the Cucurbitaceae family and an important crop. However, its yield is primarily affected by viruses. Cucumber mosaic virus (CMV) is particularly significant due to its broad host range, capable of infecting over 100 plant families. Resistance to CMV in the melon accession Songwhan Charmi (SC) is controlled by the recessive gene cmv1, which encodes the Vacuolar Protein Sorting 41, involved in vesicle transport to the vacuole. cmv1 restricts the virus to the bundle sheath cells and impedes viral access to the phloem, preventing a systemic infection. This phenotype depends on the viral movement protein (MP). However, little is known about the broader cellular changes that CMV triggers in melon or the specific biological responses that facilitate or restrict the virus entry into the phloem in susceptible and resistant varieties.ResultWe profiled the proteomes of CMV-resistant or susceptible melon genotypes inoculated with CMV-LS or FNY strains. Analysis of co-abundance networks revealed the rewiring of central biological pathways during different stages of CMV infection. Upon inoculation, resistant varieties do not trigger any signalling event to the new leaves. Local infection triggers a general depletion in proteins related to translation, photosynthesis and intracellular transport, whereas only in resistant varieties CMV triggers an increase in lipid modification and phloem proteins. During the systemic infection of susceptible melon plants, there is a strong increase in proteins associated with stress responses, such as those involved in the ER-associated degradation (ERAD) and phenylpropanoid pathways, along with a decrease in translation and photosynthesis. Key hub proteins have been identified in these processes.ConclusionsThis study is the first comprehensive high-throughput proteomic analysis of CMV-infected melon plants, providing a novel and detailed understanding of the proteomic changes associated with CMV infection, highlighting the differential responses between resistant and susceptible genotypes and identifying key proteins that could be potential targets for future research and CMV management strategies.

Keywords

CmvCmv1-mediated resistanceCucumis meloDiseaseGeneIdentificationInductionMovement proteinProteomeReplicationResistanceTobaccoTransport

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Bmc Plant Biology due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2025, it was in position 33/273, thus managing to position itself as a Q1 (Primer Cuartil), in the category Plant Sciences.

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-12-07:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 8.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 8 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 9.
  • The number of mentions on the social network X (formerly Twitter): 8 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
  • Assignment of a Handle/URN as an identifier within the deposit in the Institutional Repository: http://hdl.handle.net/20.500.12327/4555

Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: Germany.

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (Real, N) and Last Author (MARTIN HERNANDEZ, ANA MONTSERRAT).

the author responsible for correspondence tasks has been MARTIN HERNANDEZ, ANA MONTSERRAT.

Observations

MICIU/Programa Estatal de I+D+I orientada a los retos de la sociedad/RTI2018-097665-B-C21/ES/APROXIMACIONES DE VARIABILIDAD NATURAL Y EDICIÓN GENÉTICA PARA EL ESTUDIO DE LA CALIDAD DE FRUTO Y LA RESISTENCIA A ENFERMEDADES EN MELÓN/; MICINN/Programa Estatal para impulsar la investigación científico-técnica y su transferencia/PID2021-125998OB-C21/ES/Natural variability and gene editing to decipher fruit quality, disease resistance, fruit morphology and reproductive barriers in melon/; MICIU/Programa Estatal de generación del conocimiento y fortalecimiento científico y tecnológico del sistema I+D+I/CEX2019-000902-S/ES/ /

Awards linked to the item

This work was supported by the grants RTI2018-097665-B-C21 and PID2021-125998OB-C21, funded by MCIN/AEI/10.13039/501100011033, CEX2019-000902-S funded by MICIU/AEI/10.13039/501100011033 through the “Severo Ochoa Program for Centres of Excellence in R&D” and the CERCA Program/Generalitat de Catalunya.