{rfName}
Ch

Indexado en

Licencia y uso

Altmetrics

Grant support

Authors are grateful to the Spanish Government for its financial support with the project AGL2011-30519-CO3-03, and for the scholarship BES-2009-027752 (G. Buron-Moles). The authors are indebted to Dr. Pablo Librado from 'Departament de Genetica. UB' for the bioinformatician support and to Celia Sanchez from 'IRTA' for her excellent technical assistance. We also would like to thank Dr. Isabel Sanchez and Dr. M. Alba Sorolla from the 'Servei de Genomica i Proteomica; UdL' for helping on peptide mass fingerprinting and MS-MS. Erik Burchard is acknowledged for his assistance in conducting the RT-qPCR experiments.

Análisis de autorías institucional

Buron-Moles, GAutor o CoautorTeixido, NAutor o CoautorUsall, JAutor o CoautorTorres, RAutor (correspondencia)

Compartir

20 de septiembre de 2017
Publicaciones
>
Artículo
No

Characterizing the proteome and oxi-proteome of apple in response to a host (Penicillium expansum) and a non-host (Penicillium digitatum) pathogen

Publicado en:Journal Of Proteomics. 114 136-151 - 2015-01-30 114(), DOI: 10.1016/j.jprot.2014.11.007

Autores: Buron-Moles, Gemma; Wisniewski, Michael; Vinas, Inmaculada; Teixido, Neus; Usall, Josep; Droby, Samir; Torres, Rosario

Afiliaciones

ARO, Volcani Ctr, Dept Postharvest Sci, IL-50250 Bet Dagan, Israel - Autor o Coautor
IRTA, XaRTA Postharvest, Lleida 25198, Catalonia, Spain - Autor o Coautor
Lleida Univ, Dept Food Technol, XaRTA Postharvest, Agrotecnio Ctr, Lleida 25198, Catalonia, Spain - Autor o Coautor
Postcollita. IRTA Investigación y Tecnología Agroalimentarias - Autor o Coautor
Producció Vegetal. IRTA Investigación y Tecnología Agroalimentarias - Autor o Coautor
USDA ARS, Appalachian Fruit Res Stn, Kearneysville, WV 25430 USA - Autor o Coautor
Ver más

Resumen

Apples are subjected to both abiotic and biotic stresses during the postharvest period, which lead to large economic losses worldwide. To obtain biochemical insights into apple defense response, we monitored the protein abundance changes (proteome), as well as the protein carbonyls (oxi-proteome) formed by reactive oxygen species (ROS) in 'Golden Smoothee' apple in response to wounding, Penicillium expansum (host) and Penicillium digitatum (non-host) pathogens with select transcriptional studies. To examine the biological relevance of the results, we described quantitative and oxidative protein changes into the gene ontology functional categories, as well as into de KEGG pathways. We identified 26 proteins that differentially changed in abundance in response to wounding, P. expansum or P. digitatum infection. While these changes showed some similarities between the apple responses and abiotic and biotic stresses, Mal d 1.03A case, other proteins as Mal d 1.03E and EF-Tu were specifically induced in response to P. digitatum infection. Using a protein carbonyl detection method based on fluorescent Bodipy, we detected and identified 27 oxidized proteins as sensitive ROS targets. These ROS target proteins were related to metabolism processes, suggesting that this process plays a leading role in apple fruit defense response against abiotic and biotic stresses. ACC oxidase and two glutamine synthetases showed the highest protein oxidation level in response to P. digitatum infection. Documenting changes in the proteome and, specifically in oxi-proteome of apple can provide information that can be used to better understand how impaired protein functions may affect apple defense mechanisms. Possible mechanisms by which these modified proteins are involved in fruit defense response are discussed.Mechanical damage in apple fruits is linked annually to large economic losses due to opportunistic infection by postharvest pathogens, such as P. expansum. Despite the current use of chemical fungicides and the implementation of new alternative strategies, blue mold remains a critical disease of these stored fruits worldwide. Actual trends are focused on acquiring the knowledge of the host-pathogen interactions because it may help on finding new rational and environmentally friendly control alternatives. Despite the economic importance of some postharvest diseases, proteomics has only been applied in a few cases to study fruit-pathogen interactions. On the one hand, this is the first study that monitored changes at the proteome and oxi-proteome level in 'Golden Smoothee' apple fruits in response to P. expansum (compatible) and P. digitatum (non-host) pathogens. On the other hand, the main technological innovation of the reported research is the detection and quantification of oxidized (carbonylated) proteins to assess protein oxidative damage, avoiding the immunoblotting technique. The importance of the biological process investigated lies in the different mechanisms induced in fruit in response to P. expansum and P. digitatum. Results revealed that fruit recognizes and reacts to P. expansum in a similar manner to wounding, while its response to P. digitatum exhibits few differences in the protein profile. Documenting changes in the proteome and, specifically in oxi-proteome of apple can provide information that can be used to better understand how impaired protein functions may affect apple defense mechanisms. It also provides new biomarkers for oxidative damage mainly caused by the oxidative response occurring in fruit tissue in response to a host and a non-host pathogen.Copyright © 2014 Elsevier B.V. All rights reserved.

Palabras clave

blue moldexpressionfruitgene familygreen moldhydrogen-peroxideinfectionmalus domesticaoxidative stressprotein oxidationresistancert-qpcrsalicylic-acidtranscription factorBlue moldDefense-related proteinsElectrophoresis, gel, two-dimensionalFruitGreen moldHost specificityHost-pathogen interactionsMalusMalus domesticaOxidation-reductionOxidative stressPenicilliumPlant diseasesPlant proteinsProtein oxidationProteomeReactive oxygen speciesRt-qpcrStress, physiological

Indicios de calidad

Impacto bibliométrico. Análisis de la aportación y canal de difusión

El trabajo ha sido publicado en la revista Journal Of Proteomics debido a la progresión y el buen impacto que ha alcanzado en los últimos años, según la agencia WoS (JCR), se ha convertido en una referencia en su campo. En el año de publicación del trabajo, 2015, se encontraba en la posición 15/77, consiguiendo con ello situarse como revista Q1 (Primer Cuartil), en la categoría Biochemical Research Methods.

Desde una perspectiva relativa, y atendiendo al indicador del impacto normalizado calculado a partir del Field Citation Ratio (FCR) de la fuente Dimensions, arroja un valor de: 2.14, lo que indica que, de manera comparada con trabajos en la misma disciplina y en el mismo año de publicación, lo ubica como trabajo citado por encima de la media. (fuente consultada: Dimensions Jul 2025)

De manera concreta y atendiendo a las diferentes agencias de indexación, el trabajo ha acumulado, hasta la fecha 2025-07-07, el siguiente número de citas:

  • WoS: 24
  • Scopus: 14
  • Europe PMC: 5

Impacto y visibilidad social

Desde la dimensión de Influencia o adopción social, y tomando como base las métricas asociadas a las menciones e interacciones proporcionadas por agencias especializadas en el cálculo de las denominadas “Métricas Alternativas o Sociales”, podemos destacar a fecha 2025-07-07:

  • La utilización de esta aportación en marcadores, bifurcaciones de código, añadidos a listas de favoritos para una lectura recurrente, así como visualizaciones generales, indica que alguien está usando la publicación como base de su trabajo actual. Esto puede ser un indicador destacado de futuras citas más formales y académicas. Tal afirmación es avalada por el resultado del indicador “Capture” que arroja un total de: 76 (PlumX).

Análisis de liderazgo de los autores institucionales

Este trabajo se ha realizado con colaboración internacional, concretamente con investigadores de: Israel; United States of America.

Existe un liderazgo significativo ya que algunos de los autores pertenecientes a la institución aparecen como primer o último firmante, se puede apreciar en el detalle: Primer Autor (BURON MOLES, GEMMA) y Último Autor (TORRES SANCHIS, ROSARIO).

el autor responsable de establecer las labores de correspondencia ha sido TORRES SANCHIS, ROSARIO.