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Analysis of institutional authors

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June 7, 2023
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Impact of RNA Degradation on Viral Diagnosis: An Understated but Essential Step for the Successful Establishment of a Diagnosis Network

Publicated to: Veterinary Sciences . 5 (1): 19- - 2018-03-01 5(1), DOI: 10.3390/vetsci5010019

Authors: Relova, Damarys; Rios, Liliam; Acevedo, Ana M; Coronado, Liani; Perera, Carmen L; Perez, Lester J

Affiliations

Ctr Nacl Sanidad Agr CENSA, OIE Collaborating Ctr Diag & Risk Anal Caribbean, Havana 32700, Cuba - Author
Dalhousie Univ, Dalhousie Med New Brunswick, St John, NB E2L 4L5, Canada - Author
Univ New Brunswick, Fac Med, Reiman Canc Res Lab, St John, NB E2L 4L5, Canada - Author
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Abstract

The current global conditions, which include intensive globalization, climate changes, and viral evolution among other factors, have led to an increased emergence of viruses and new viral diseases; RNA viruses are key drivers of this evolution. Laboratory networks that are linked to central reference laboratories are required to conduct both active and passive environmental surveillance of this complicated global viral environment. These tasks require a continuous exchange of strains or field samples between different diagnostic laboratories. The shipment of these samples on dry ice represents both a biological hazard and a general health risk. Moreover, the requirement to ship on dry ice could be hampered by high costs, particularly in underdeveloped countries or regions located far from each other. To solve these issues, the shipment of RNA isolated from viral suspensions or directly from field samples could be a useful way to share viral genetic material. However, extracted RNA stored in aqueous solutions, even at -70 degrees C, is highly prone to degradation. The current study evaluated different RNA storage conditions for safety and feasibility for future use in molecular diagnostics. The in vitro RNA-transcripts obtained from an inactivated highly pathogenic avian influenza (HPAI) H5N1 virus was used as a model. The role of secondary structures in the protection of the RNA was also explored. Of the conditions evaluated, the dry pellet matrix was best able to protect viral RNA under extreme storage conditions. This method is safe, cost-effective and assures the integrity of RNA samples for reliable molecular diagnosis. This study aligns with the globally significant Global One Health paradigm, especially with respect to the diagnosis of emerging diseases that require confirmation by reference laboratories.

Keywords

laboratory networksreal time rt-pcrrna storagesample exchangesecondary structuresLaboratory networksReal time rt-pcrRna storageSample exchangeSecondary structuresViral diagnosis

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Veterinary Sciences due to its progression and the good impact it has achieved in recent years, according to the agency Scopus (SJR), it has become a reference in its field. In the year of publication of the work, 2018, it was in position , thus managing to position itself as a Q2 (Segundo Cuartil), in the category Veterinary (Miscellaneous).

Independientemente del impacto esperado determinado por el canal de difusión, es importante destacar el impacto real observado de la propia aportación.

Según las diferentes agencias de indexación, el número de citas acumuladas por esta publicación hasta la fecha 2025-12-14:

  • WoS: 33
  • Europe PMC: 27

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-12-14:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 92.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 92 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 1.
  • The number of mentions on the social network X (formerly Twitter): 1 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.

Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: Canada; Cuba.