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This work had been funded by Spanish Government Ministerio de Ciencia e Innovacion Agencia Estatal de Investigacion (AEI) (project PID2019-103985RR-I00); Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria (INIA) (projects RTA2015-00027-00-00 and RFP2015-00015-00-00); and FEDER funds. Addiccional funding was obtained from Grupo de Investigacion de la Comunidad de Aragon A12-17R (Fruticultura. Caracterizacion, Adaptacion y Mejora Genetica) of Departamento de Innovacion, Investigacion y Universidad (Gobierno de Aragon). AC was funded by Departamento de Innovacion, Investigacion y Universidad, Gobierno de Aragon, and by the Ph.D. program Subvenciones destinadas a la contratacion de personal investigador en formacion 2015-2019.

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Article

Identification and Characterization of DAMs Mutations Associated With Early Blooming in Sweet Cherry, and Validation of DNA-Based Markers for Selection

Publicated to:Frontiers In Plant Science. 12 621491- - 2021-07-08 12(), DOI: 10.3389/fpls.2021.621491

Authors: Calle, Alejandro; Grimplet, Jerome; Le Dantec, Loick; Wunsch, Ana

Affiliations

Ctr Invest & Tecnol Agroalimentaria Aragon, Unidad Hortofruticultura, Zaragoza, Spain - Author
Univ Bordeaux, INRAE, Biol Fruit & Pathol, UMR 1332, Villenave Dornon, France - Author
Univ Zaragoza, Inst Agroalimentario Aragon IA2, CITA, Zaragoza, Spain - Author

Abstract

Dormancy release and bloom time of sweet cherry cultivars depend on the environment and the genotype. The knowledge of these traits is essential for cultivar adaptation to different growing areas, and to ensure fruit set in the current climate change scenario. In this work, the major sweet cherry bloom time QTL qP-BT1.1(m) (327 Kbs; Chromosome 1) was scanned for candidate genes in the Regina cv genome. Six MADS-box genes (PavDAMs), orthologs to peach and Japanese apricot DAMs, were identified as candidate genes for bloom time regulation. The complete curated genomic structure annotation of these genes is reported. To characterize PavDAMs intra-specific variation, genome sequences of cultivars with contrasting chilling requirements and bloom times (N = 13), were then mapped to the 'Regina' genome. A high protein sequence conservation (98.8-100%) was observed. A higher amino acid variability and several structural mutations were identified in the low-chilling and extra-early blooming cv Cristobalina. Specifically, a large deletion (694 bp) upstream of PavDAM1, and various INDELs and SNPs in contiguous PavDAM4 and -5 UTRs were identified. PavDAM1 upstream deletion in 'Cristobalina' revealed the absence of several cis-acting motifs, potentially involved in PavDAMs expression. Also, due to this deletion, a non-coding gene expressed in late-blooming 'Regina' seems truncated in 'Cristobalina'. Additionally, PavDAM4 and -5 UTRs mutations revealed different splicing variants between 'Regina' and 'Cristobalina' PavDAM5. The results indicate that the regulation of PavDAMs expression and post-transcriptional regulation in 'Cristobalina' may be altered due to structural mutations in regulatory regions. Previous transcriptomic studies show differential expression of PavDAM genes during dormancy in this cultivar. The results indicate that 'Cristobalina' show significant amino acid differences, and structural mutations in PavDAMs, that correlate with low-chilling and early blooming, but the direct implication of these mutations remains to be determined. To complete the work, PCR markers designed for the detection of 'Cristobalina' structural mutations in PavDAMs, were validated in an F-2 population and a set of cultivars. These PCR markers are useful for marker-assisted selection of early blooming seedlings, and probably low-chilling, from 'Cristobalina', which is a unique breeding source for these traits.

Keywords

bloomingbreedingcandidate geneschill requirementchilling requirementdamsdormancyexpressiongene expression regulationheat requirementsnon-coding genepeachpersica l. batschphenological traitspyrus-pyrifoliautrsBloomingBreedingChill requirementDamsGene expression regulationMads-box genesNon-coding genePrunus avium lUtrs

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Frontiers In Plant Science due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2021, it was in position 20/239, thus managing to position itself as a Q1 (Primer Cuartil), in the category Plant Sciences. Notably, the journal is positioned above the 90th percentile.

From a relative perspective, and based on the normalized impact indicator calculated from the Field Citation Ratio (FCR) of the Dimensions source, it yields a value of: 3.3, which indicates that, compared to works in the same discipline and in the same year of publication, it ranks as a work cited above average. (source consulted: Dimensions Jun 2025)

Specifically, and according to different indexing agencies, this work has accumulated citations as of 2025-06-13, the following number of citations:

  • WoS: 10
  • Scopus: 13
  • Europe PMC: 8
  • OpenCitations: 11

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-06-13:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 16.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 16 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 0.75.
  • The number of mentions on the social network X (formerly Twitter): 2 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.

Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: France.

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (CALLE CALDERON, ALEJANDRO) .