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This work was performed with the financial support of the CIEN project BIOPRO from "Centro para el Desarrollo Tecnologico Industrial" (CDTI), Ministry of Science and Innovation, Government of Spain.

Analysis of institutional authors

Pera, JAuthorParlade, JAuthorCastellari, MAuthor

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December 5, 2021
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Article

Grifola frondosa (Maitake) Extract Reduces Fat Accumulation and Improves Health Span in C. elegans through the DAF-16/FOXO and SKN-1/NRF2 Signalling Pathways

Publicated to:Nutrients. 13 (11): 3968- - 2021-11-01 13(11), DOI: 10.3390/nu13113968

Authors: Aranaz, Paula; Pena, Adriana; Vettorazzi, Ariane; Fabra, Maria Jose; Martinez-Abad, Antonio; Lopez-Rubio, Amparo; Pera, Joan; Parlade, Javier; Castellari, Massimo; Milagro, Fermin, I; Gonzalez-Navarro, Carlos J

Affiliations

2017 SGR 1618 Protecció Vegetal Sostenible. IRTA Investigación y Tecnología Agroalimentarias - Author
2017 SGR 1650 SEQUSAL. IRTA Investigación y Tecnología Agroalimentarias - Author
Funcionalitat i Seguretat Alimentària. IRTA Investigación y Tecnología Agroalimentarias - Author
Indústries Alimentàries. IRTA Investigación y Tecnología Agroalimentarias - Author
Inst Agr & Food Res & Technol IRTA, Finca Camps & Armet S-N, Monells 17121, Spain - Author
Inst Agrochem & Food Technol IATA CSIC, Food Safety & Preservat Dept, Valencia 46980, Spain - Author
Inst Salud Carlos III, Ctr Invest Biomed Red Fisiopatol Obesidad & Nutr, Madrid 28029, Spain - Author
IRTA, Inst Agr & Food Res & Technol IRTA, Cabrils Ctr, Ctra Cabrils Km 2, Barcelona 08348, Spain - Author
Navarra Inst Hlth Res IdiSNa, Pamplona 31008, Spain - Author
Producció Vegetal. IRTA Investigación y Tecnología Agroalimentarias - Author
Protecció Vegetal Sostenible . IRTA Investigación y Tecnología Agroalimentarias - Author
Spanish Natl Res Council SusPlast CSIC, Interdisciplinary Platform Sustainable Plast Circ, Madrid 28006, Spain - Author
Univ Navarra, Ctr Nutr Res, Pamplona 31008, Spain - Author
Univ Navarra, Sch Pharm & Nutr, Dept Pharmacol & Toxicol, MITOX Res Grp, Pamplona 31008, Spain - Author
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Abstract

In recent years, food ingredients rich in bioactive compounds have emerged as candidates to prevent excess adiposity and other metabolic complications characteristic of obesity, such as low-grade inflammation and oxidative status. Among them, fungi have gained popularity for their high polysaccharide content and other bioactive components with beneficial activities. Here, we use the C. elegans model to investigate the potential activities of a Grifola frondosa extract (GE), together with the underlying mechanisms of action. Our study revealed that GE represents an important source of polysaccharides and phenolic compounds with in vitro antioxidant activity. Treatment with our GE extract, which was found to be nongenotoxic through a SOS/umu test, significantly reduced the fat content of C. elegans, decreased the production of intracellular ROS and aging-lipofuscin pigment, and increased the lifespan of nematodes. Gene expression and mutant analyses demonstrated that the in vivo anti-obesity and antioxidant activities of GE were mediated through the daf-2/daf-16 and skn-1/nrf-2 signalling pathways, respectively. Taken together, our results suggest that our GE extract could be considered a potential functional ingredient for the prevention of obesity-related disturbances.

Keywords

bioactive compoundsfood ingredientsinsulinmetabolic syndromenutraceutical fungiAdipose tissueAdipose-tissueAgingAnimalsAnti-obesity agentsAntioxidantAntioxidantsBioactive compoundsCaenorhabditis elegansCaenorhabditis elegans proteinsCaenorhabditis-elegansComplex mixturesDaf-16 protein, c elegansDietary supplementsDna-binding proteinsFood ingredientsForkhead transcription factorsFrondosaFruit bodyGrifolaInsulinLipofuscinLongevityMetabolic syndromeNf-e2-related factor 2Nutraceutical fungiObesityOxidative stressPolysaccharidesReactive oxygen speciesResistanceSignal transductionSkn-1 protein, c elegansSos/umu-testTranscription factors

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Nutrients due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2021, it was in position 15/90, thus managing to position itself as a Q1 (Primer Cuartil), in the category Nutrition & Dietetics.

From a relative perspective, and based on the normalized impact indicator calculated from the Field Citation Ratio (FCR) of the Dimensions source, it yields a value of: 5.6, which indicates that, compared to works in the same discipline and in the same year of publication, it ranks as a work cited above average. (source consulted: Dimensions Jul 2025)

Specifically, and according to different indexing agencies, this work has accumulated citations as of 2025-07-17, the following number of citations:

  • WoS: 15
  • Europe PMC: 5

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-07-17:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 52.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 53 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 2.5.
  • The number of mentions on the social network Facebook: 1 (Altmetric).
  • The number of mentions on the social network X (formerly Twitter): 3 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
  • Assignment of a Handle/URN as an identifier within the deposit in the Institutional Repository: http://hdl.handle.net/20.500.12327/1566