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Grant support

This research was funded by the Ministerio de Ciencia e Innovacion (MICIN) and the Agencia Estatal de Investigacion (AEI) through the CELLECTRA (PID2020-112976RB-C21 and PID2020-112976RB-C22) project and by the European Commission through the BLUESHELL- FISH (HORIZON-MSCA-101086234) project. Mounira Alkassar acknowledges MICIN and AEI for her PhD grant (PRE2019-088181) . Jaume Reverte acknowledges IRTA for his PhD grant (CPI0422) . The authors also acknowledge support from CERCA Program/Generalitat de Catalunya.

Analysis of institutional authors

Alkassar, MouniraAuthorReverté, JaumeAuthorDiogene, JorgeAuthorCampas, MonicaCorresponding Author

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Article

β-cyclodextrin polymer as tetrodotoxins scavenger in oyster extracts

Publicated to:Microchemical Journal. 201 110585- - 2024-06-01 201(), DOI: 10.1016/j.microc.2024.110585

Authors: Alkassar, Mounira; Reverte, Jaume; Fragoso, Alex; Torrens, Mabel; Klijnstra, Mirjam; Gerssen, Arjen; Diogene, Jorge; Campas, Monica

Affiliations

Aigües Marines i Continentals. IRTA Investigación y Tecnología Agroalimentarias - Author
IRTA, Ctra Poble Nou Km 5-5, La Rapita 43540, Spain - Author
Producció Animal. IRTA Investigación y Tecnología Agroalimentarias - Author
Univ Rovira & Virgili, Ave Paisos Catalans 26, Tarragona 43007, Spain - Author
Wageningen Univ & Res, Wageningen Food Safety Res, Akkermaalsbos 2, NL-6708 WB Wageningen, Netherlands - Author
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Abstract

Tetrodotoxins (TTXs) are potent marine neurotoxins involved in poisoning cases in humans after consumption of some marine organisms. A cell-based assay (CBA) providing toxicological information is a commonly used method for their detection because of its high sensitivity. However, matrix effects may interfere in the CBA. In this work, five insoluble cyclodextrin polymers, with different chemicals structures (beta CDP-14-DS2, beta CDP-13-DS2, beta CDP-13-DS4, beta CDP-14-DS4 and beta CDP-N-DS5) have been investigated as novel clean-up materials for oyster extracts containing TTXs. The best recoveries have been achieved with beta CDP-14-DS2 (similar to 100 %), which allowed exposing cells to 27-fold higher oyster matrix concentrations and decreased the LOQ down to 46 mu g equiv. TTX/kg. Then, the applicability of this strategy has been demonstrated with oyster extracts from The Netherlands and comparing the results with immunoassay and liquid chromatography-tandem mass spectrometry (LC-MS/MS). This clean-up strategy combined with CBA could be implemented for TTXs detection in monitoring programs to ensure seafood safety.

Keywords

Cell-based assayClean-upCyclodextrin polymersFisFood safetMatrix effectNeuroblastomaQuantitationSaxitoxinShellfishTetrodotoxinsToxins

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Microchemical Journal due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2024 there are still no calculated indicators, but in 2023, it was in position 19/106, thus managing to position itself as a Q1 (Primer Cuartil), in the category Chemistry, Analytical.

Independientemente del impacto esperado determinado por el canal de difusión, es importante destacar el impacto real observado de la propia aportación.

Según las diferentes agencias de indexación, el número de citas acumuladas por esta publicación hasta la fecha 2025-07-02:

  • WoS: 1

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-07-02:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 6.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 6 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 0.75.
  • The number of mentions on the social network X (formerly Twitter): 2 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
  • Assignment of a Handle/URN as an identifier within the deposit in the Institutional Repository: http://hdl.handle.net/20.500.12327/2951

Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: Netherlands.

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (ALKASSAR, MOUNIRA) and Last Author (CAMPAS HOMS, MONICA).

the author responsible for correspondence tasks has been CAMPAS HOMS, MONICA.